Specifications include, but are not limited to: The system should be automated for both real-time PCR and post-PCR (end point) analysis using in-built ARIA Software. System should support applications including absolute quantitation, simultaneous analysis data for relative quantitation of Unlimited plates of 96 wells each, and Configurable Optical Modules Instrument should have 96 well sample block of 0.1ml capacity, with a maximum block ramp rate of 6 °C/Sec System should complete Fast 40 cycle protocol in less than 40 minutes and standard protocol in under 2 hours. The system should have LCD touch display with USB interface to export the data. The temperature range of the thermoblock should be 4°C to 99°C with accuracy of ±0.25°C The system should have a minimum 8 filtered photodiodes The Excitation source should have 8 dye specific LEDs per optical module and emission detection by 8 filtered photodiodes. There should be enough excitation and emission filters to cover majority of dyes. System software should provide simultaneous analysis data for relative quantitation of Unlimited plates of 96 wells each. The system should have Normalization of reaction due to non-PCR related fluctuations such as pipetting variations. System should support reaction volume 2-30 μL. All assays should run using Universal Thermal Cycling conditions to eliminate for the Optimisation of PCR machine.
