Specifications include, but are not limited to:2.1 TRIPLE QUADRUPOLE MASS SPECTROMETER SPECIFICATIONS The triple quadrupole mass spectrometer must meet, at minimum, the following specifications: A. Quickly switch polarity (<25ms) in ESI (electrospray ionization) mode within the same analytical run. B. Analyze a mass range of m/z 5-3000. C. Minimal mass accuracies: 1. +/- 0.1 Da from m/z 5 to 1,000. 2. +/- 0.2 Da from m/z 1,000 to 2,000. 3. +/- 0.3 Da from m/z 2,000 to 3,000. D. Operate in MRM (multiple reaction monitoring), SIM (secondary ion mass), MS Scan, product ion scan and precursor ion scan. E. Analyze 500 MRMs/sec. F. Analyze pesticides at trace level (1ug/kg) in difficult matrices. G. Mass resolution (autotune), full width at half maximum: 0.7 Da (in Narrow mode). H. Mass stability <0.1 Da drift in 24 hours up to m/z 2,122 in positive mode and m/z 2,234 in negative mode. I. Linear dynamic range of >6.0 x 106. J. Triple quadrupole (QQQ) modes of operations MRM (including dynamic and triggered), SIM, MS Scan, product ion scan, neutral loss/gain scan and precursor ion scan. K. Maximum scan rate 17,000Da/s. L. Maximum MRM acquisition rate 500 MRMs/s. M. Minimum MRM dwell time 0.5ms. N. Orthogonal ionization sources (must include wide range): 1. Electrospray (ESI). 2. Nanospray ESI-for low applications 3. APCI source (Atmospheric Pressure Chemical Ionization). 4. Multimode source (simultaneous ESI and APCI). O. Reinjection artificial intelligence-intelligent and automated secondary reinjection logic. P. Solvent declustering-countercurrent drying gas and heated nebulizer sheath gas on standard ion source. Q. Detector - +/- 20kV high-energy conversion dynode (HED) and high-gain electron multiplier horn. R. Vacuum system-two turbomolecular pumps with one mechanical pump. S. VacShield Assembly-vent prevention. T. PFAS MRM Library-curated database of ~ 100 native and isotopically labelled PFAS compounds and MS acquisition parameters U. Pesticide MRM Library – a library curated with most common use pesticides and their MS acquisition parameters. Page 6 of 13 V. The triple quadrupole mass spectrometer must meet the following MRM (multiple reaction monitoring) sensitivity requirements: 1. Signal-to-Noise ratio (S/N), ESI (electrospray ionization) positive-1 pg of reserpine injected on column, quantifying on the transition m/z 609.3 to 195.1:S/N>1,200,000:1. 2. Signal-to-Noise ratio (S/N), ESI (electrospray ionization) negative-1 pg of chloramphenicol injected on column, quantifying on the transition m/z 321.0 to 152.0:S/N>1,200,000:1. 3. Instrument Detection Limit (IDL), ESI (electrospray ionization) positive-1 fg of reserpine injected on column, quantifying on the transition m/z 609.3 to 195.1:IDL<0.6 fg with 99% confidence. 4. Instrument Detection Limit (IDL), ESI (electrospray ionization) Negative-1 fg of chloramphenicol injected on column, quantifying on the transition m/z 321.0 to 152.0:IDL<0.6 fg with 99% confidence.
