Bidder's proposal must include a high resolution respirometer that utilizes a gold cathode rather than the less sensitive platinum electrode with a diameter of 2 mm to increase the signal to noise ratio to provide accuracy in measuring slow rates of oxygen consumption (1.0 pmol O2 s-1 mL-1). MANDATORY 2.2.Bidder's proposal must include a high resolution respirometer with the ability to accurately measure rates of oxygen consumption, which is highly dependent upon temperature (± 0.002ºC) and ambient pressure (±0.1 kPa). MANDATORY 2.3.Bidder's proposal must include a high resolution respirometer with a high level of temperature control (± 0.002ºC from 4ºC to 47ºC) achieved using Peltier thermopiles and a Peltier heat dissipation plate, both in thermal contact with a copper block that surrounds the glass reaction chambers. MANDATORY 2.4.Bidder's proposal must include a high resolution respirometer that has the capability to measure oxygen consumption and fluorescence simultaneously. MANDATORY 2.5.Bidder's proposal must include a fluorescence attachment with filters for 525 nm to measure reactive oxygen species (ROS) production using Amplex Red and to measure mitochondrial membrane potential using TMRM. The fluorescence attachment must have a filter for 465 nm to measure mitochondrial membrane potential using safranin. The bidder must also have filter capabilities to measure calcium green and magnesium green for Ca2+uptake capacity (CaG), ATP production (MgG) or photobiology measurements. MANDATORY...
